ABSTRACT
The present study was to determine the prevalence of pseudomonas aeruginosa in patients using clinical samples (urine, skin lesion and pus) and environmental samples (sand, tap water, flowers). A total of thirty (30) samples were collected from patients, ten (10) each of urine, skin lesions and pus. And also three environmental samples (tap water, sand and flower) were collected. The samples obtained were cultured using standard microbiological methods and the isolates obtained were identified based on their colonial morphology, motility testing, gram staining reaction and biochemical tests. The antimicrobial sensitivity pattern of the isolates to ten (10) different antibiotics was determined. The results obtained showed that the micro organisms seen in the sample were staphylococcus spp, Escherichia spp, Klebsiella spp, proteus spp and Pseudomonas aeruginosa. The environment samples were negative for Pseudomonas aeruginosa but staphylococcus spp and Escherichia spp were seen. The percentage prevalence of the isolated organism from the environment samples was 50% in each. The prevalence (%) of p. aeruginosa isolated from clinical samples were 12.5%(urine), 31.25%(lesion) and 15.38%(pus). The results were also determined according to sex for each sample. For male, urine (6.25%), skin lesion (18.75%) and pus (7.69%) also for female, urine (6.25%), skin lesions (12.5%) and pus (7.69%). The presence of Pseudomonas aeruginosa is of public health significance and these findings indicates the prevalence of Pseudomonas aeruginosa, thereby providing clues to prevention and treatments.
TABLE OF CONTENTS
Title Page i
Certification ii
Dedication iii
Acknowledgement iv
Abstract v
Table of contents vi
CHAPTER ONE
1.0 Introduction 1
1.1 Justification 3
1.2 Aims and Objectives 4
CHAPTER TWO
LITERATURE REVIEW
2.1 Classification History of Pseudomonas 6
2.2 Pseudomonas group and their characteristics 7
2.3 Epidemiology of Pseudomonas Aeruginosa 9
2.4 Cultural Characteristics 12
2.5 Identification of Pseudomonas Aeruginosa 12
2.6 Pathogenesis 17
2.7 Signs and Symptoms 24
2.8 Laboratory Diagnosis 30
2.9 Other Method of Diagnosing Pseudomonas Aeruginosa 31
2.10 Treatment 33
2.11 Prevention and Control 36
CHAPTER THREE
MATERIALS AND METHODS
3.1 Study Area 38
3.2 Method 39
3.3 Samples Collection and Processing 40
3.4 Viable Count 43
3.5 Isolation and Identification 43
3.5.1Biochemical Test 45
CHAPTER FOUR
Results 48
CHAPTER FIVE
5.1 Discussion 65
5.2 Conclusion and Recommendation 68
References 70
Appendix 80
